Sample Requirements

  • Must contain viability marker (103Rh, 194Pt, cisPt, or 198Pt).
  • Must be fixed with minimum 1.6% PFA.
  • Must contain Ir191/193 for cell identification.
  • Sample concentration: 10,000 - 1,000,000 cells/ml.
  • FILTERED through a 35um filter.
  • Must be resuspended in 1:5 - 1:10 EQ4 Beads and Cell Acquisition Solution. 
  • Be in 5ml FACs or Eppendorf tubes.

​For novel projects please contact us prior to preparing your samples

Shipped Samples

The CyTOF core accepts shipped samples!  
  • Samples must be stained by the researcher.
  • Shipped overnight on ice in either the DNA intercalator step or as a pellet in 4%PFA prior to DNA intercalator.
  • The package must contain a list of the samples with cell counts and a copy of the antibody panel.  
  • Reservations for shipped samples should be made no earlier than 12:00pm on the day the sample is scheduled to arrive.  
  • Researchers should give the Core staff the tracking for the shipment.
  • Core staff will  perform the DNA intercalator (if necessary), final washes, and resuspension/filtering steps one hour prior to the scheduled reservation time.  
  • Technician time for final sample prep is charged at the consultation rate: 1 hour for samples shipped in DNA intercalator or 1.5 hours for samples shipped as a pellet prior to DNA intercalator.

Attn: Noa Schork / CyTOF Core MA309
Dana-Farber Cancer Institute
1 Jimmy Fund Way
Boston, MA 02215
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