Sample Requirements

  • Sample concentration: 10,000 - 1,000,000 cells/ml
  • Must be FIXED with minimum 1.6% PFA
  • FILTERED through a 35um filter
  • Must be in WATER or Cell Acquisition Solution
  • Must contain Ir191/193 for cell identification
  • Must contain viability marker (103Rh, 194Pt, cisPt, or 198Pt)
  • Must contain 4-element EQ Beads at 1:10 for normalization
  • Sample time: 500ul = 20 minutes
  • Be in 5ml FACs or Eppendorf tubes (if running on the plate loader, samples will be transferred to the plate upon arrival

For novel projects please contact us prior to preparing your samples
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