Sample Requirements

• Sample concentration: 10,000 - 1,000,000 cells/ml
• Must be FIXED with minimum 1.6% PFA
• FILTERED through a 35um filter
• Must be in WATER or Cell Acquisition Solution
• Must contain Ir191/193 for cell identification
• Must contain viability marker (103Rh, 194Pt, cisPt, or 198Pt)
• Must contain 4-element EQ Beads at 1:10 for normalization
• Sample time: 500ul = 20 minutes
• Be in 5ml FACs or Eppendorf tubes

For novel projects please contact us prior to preparing your samples

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