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Mass Cytometry Core

Longwood Medical Area CyTOF
Mass Cytometry Core

Sample Requirements

  • Sample concentration: 10,000 - 1,000,000 cells/ml
  • Must be FIXED with minimum 1.6% PFA
  • FILTERED through a 35um filter
  • Must be in WATER or Cell Acquisition Solution
  • Must contain Ir191/193 for cell identification
  • Must contain viability marker (103Rh, 194Pt, cisPt, or 198Pt)
  • Must contain 4-element EQ Beads at 1:10 for normalization
  • Sample time: 500ul = 20 minutes
  • Be in 5ml FACs or Eppendorf tubes
For novel projects please contact us prior to preparing your samples
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