Sample Requirements

  • Sample concentration: 10,000 - 1,000,000 cells/ml
  • Must be FIXED with minimum 1.6% PFA
  • FILTERED through a 35um filter
  • Must be in water or Cell Acquisition Solution
  • Must contain Ir191/193 for cell identification
  • Must contain viability marker (103Rh, 194Pt, cisPt, or 198Pt)
  • Must contain 4-element EQ Beads at 1:10 for normalization
  • Sample time: 500ul = 20 minutes
  • Be in 5ml FACs or Eppendorf tubes
For novel projects please contact us prior to preparing your samples

Shipped Samples

The CyTOF core can accept shipped samples!  
  • Samples must be stained by the researcher
  • Shipped overnight on ice in either the DNA intercalator step or as a pellet in 4%PFA prior to DNA intercalator
  • The package must contain a list of the samples with cell counts and a copy of the antibody panel.  
  • Reservations for shipped samples should be made no earlier then 12:00pm on the day the sample is scheduled to arrive.  
  • Core staff will  preform the DNA intercalator (if necessary), final wash and filtering steps one hour prior to the scheduled reservation time.  
  • Technician time for final sample prep is charged at the consultation rate - 1 hour for samples shipped in DNA intercalator or 1.5 hours for samples shipped as a pellet prior to DNA intercalator.

Shipping
Attn: Noa Schork / CyTOF Core
Dana-Farber Cancer Institute
450 Brookline Ave., MA309
Boston, MA 02215
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