Our Services

Consultation

Consultation is available from initial experiment setup throughout your project. Expert technical assistance regarding experimental design, acquisition, interpretation, troubleshooting and primary analysis are provided.
​

Sample Acquisition

Fully assisted sample acquisition and interpretation is provided during regular staffed hours. Post-data processing such as normalization and debarcoding, are included by request.  Data can be accessed within 24 hours of reservations on Pydio.
​
Self-run acquisition training is available to researchers requiring time on nights and weekends. 

Data Analysis Training

Our core staff  is available to assist with understanding your data and training on publicly available data analysis platforms.
Data workstations are provided at no charge for researchers to access normalization, debarcoding, FlowJo and Cytobank data software. 

Rates 

Sample Acquisition

DFCI & BWH
External Academic
Corporate
$100.00/hour
$185.00/hour
​$250.00/hour
​(rates subject to change)
  • Rates are calculated in 0.5 hour increments.
  • 1 hour minimum reservation for instrument use
  • 0.5 hour minimum reservation for consultations and bioinformatics
  • Initial instrument training requires a 4 hour reservation
  • Please see policies for information on sample requirements and cancellations
  • Samples resuspended in water will result in an extra charged hour to accommodate the time to switch injectors 

Sample Requirements

Picture
  • Sample concentration: 10,000 - 1,000,000 cells/ml
  • Must be FIXED with minimum 1.6% PFA
  • FILTERED through a 35um filter
  • Must be in water or Cell Acquisition Solution
  • Must contain Ir191/193 for cell identification
  • Must contain viability marker (103Rh, 194Pt, cisPt, or 198Pt)
  • Must contain 4-element EQ Beads at 1:10 for normalization
  • Sample time: 500ul = 20 minutes
  • Be in 5ml FACs or Eppendorf tubes
For novel projects please contact us prior to preparing your samples

Shipped Samples

The CyTOF core can accept shipped samples!  
  • Samples must be stained by the researcher
  • Shipped overnight on ice in either the DNA intercalator step or as a pellet in 4%PFA prior to DNA intercalator
  • The package must contain a list of the samples with cell counts and a copy of the antibody panel.  
  • Reservations for shipped samples should be made no earlier then 12:00pm on the day the sample is scheduled to arrive.  
  • Core staff will  preform the DNA intercalator (if necessary), final wash and filtering steps one hour prior to the scheduled reservation time.  
  • Technician time for final sample prep is charged at the consultation rate - 1 hour for samples shipped in DNA intercalator or 1.5 hours for samples shipped as a pellet prior to DNA intercalator.

Shipping
Attn: Noa Schork / CyTOF Core
Dana-Farber Cancer Institute
450 Brookline Ave., MA309
Boston, MA 02215