Fully assisted sample acquisition and interpretation is provided during regular staffed hours. Post-data processing, such as normalization and data clean up or debarcoding, are included for all reservations. Data will be shared through Partners' Secure File Share within 24 hours from the end of the reservation.
Self-run acquisition training is available to researchers requiring time on nights and weekends.
Final Sample Preparation
(For shipped samples only)
Core staff will perform the final washes (twice with Cell Staining Buffer (CSB) and twice with Cell Acquisition Solution (CAS). They will then perform a cell count, resuspend the samples in 1:5 EQ4 beads and CAS Solution, filter, and acquire on the CyTOF-Helios instrument. DNA intercalator steps will be performed if necessary.