The Longwood Medical Area CyTOF core provides high dimensional cell analysis for cellular phenotyping, phosphorylation, cytokine and receptor expression in a large variety of human and murine specimens focused in areas of systems biology, immunology, cancer research, autoimmunity, stem cell biology, drug delivery and more!
- Coming soon: two state of the art CyTOF-XT mass cytometers, featuring an auto-sampler and improved signal stability. Undergoing validation now.
- Two CyTOF-Helios mass cytometers with the ability to measure over 40 available antibody channels simultaneously with minimal signal overlap
- Support in various aspects of utilizing mass cytometry technology from initial consultation to data interpretation
Mass cytometry couples highly sensitive ICP-TOF technology with flow cytometry-like high speed single cell analysis
How it works
Cells in suspension are fixed and stained metal-conjugated antibodies. These cells are individually introduced to the ICP (inductively coupled plasma) where they are atomized and ionized to form an ion cloud. Biological elements are filtered out before the ion cloud then passes through the TOF (time-of-flight) chamber where ions are separated by mass and counted. Data is exported into .fcs files for analysis.
Antibodies are conjugated to non-rare, non-biological, non-radioactive isotopes, mostly within the lanthanide series. High isotopic purity combined with proper panel design helps ensure low signal overlap.