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Mass Cytometry Core

Longwood Medical Area CyTOF
Mass Cytometry Core

About US

The Longwood Medical Area CyTOF core provides high dimensional cell analysis for cellular phenotyping, phosphorylation, cytokine and receptor expression in a large variety of human and murine specimens focused in areas of systems biology, immunology, cancer research, autoimmunity, stem cell biology, drug delivery and more! ​

Our Resources

  • Two CyTOF-Helios mass cytometers with the ability to measure over 40 available antibody channels simultaneously with minimal signal overlap​
  • Support in various aspects of utilizing mass cytometry technology from initial consultation to data interpretation
  • Available to DFCI researchers as well as researchers from outside institutions and companies. 
  • 5+ years of experience and expertise

Mass cytometry couples highly sensitive ICP-TOF technology with flow cytometry-like high speed single cell analysis

How it works

Cells in suspension are fixed and stained metal-conjugated antibodies. These cells are individually introduced to the ICP (inductively coupled plasma) where they are atomized and ionized to form an ion cloud. Biological elements are filtered out before the ion cloud then passes through the TOF (time-of-flight) chamber where ions are separated by mass and counted. Data is exported into .fcs files for analysis. ​
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Features

  • Currently up to 42 markers measured simultaneously (135 theoretical channels)
  • Additional channels for cell identification and viability
  • Sample barcoding to multiplex up to 20 samples in a single tube
  • Minimal signal overlap (no compensation)
  • Very low biological background contamination
  • Ability to run in 5ml FACs or eppendorf tubes
  • Record up to 2 million cells per hour
  • Beads added to sample allows for data normalization to global standards
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Antibodies

Antibodies are conjugated to non-rare, non-biological, non-radioactive isotopes, mostly within the lanthanide series.  High isotopic purity combined with proper panel design helps ensure low signal overlap. ​
Resources

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©2017 Mass Cytometry Core at Dana-Farber Cancer Institute