• Must contain viability marker (103Rh, 194Pt, cisPt, or 198Pt).
• Must be FIXED with minimum 1.6% PFA.
• Metal Free BSA (Sigma)
• Must contain Ir191/193 for cell identification.​
• Must be brought in 5mL polypropylene tubes
• ​MAXIMUM of 1 million pelleted cells per tube
  
  • ​Individual or barcoded samples that exceed 1 million cells should be
    split evenly across multiple tubes (for example, a sample with 3 million cells
    should be split into 3 polypropylene tubes with 1 million cells in each tube).
• Cell Counts (including Cell Viability) obtained prior to final pelleting, must be provided to  staff
• Always perform 2 final washes using CAS Plus.
• Samples are added to the Autosampler carousel in Pellet Form ONLY
  
  • DO NOT ADD BEADS (Autosampler adds EQ6 beads and resuspends the sample in CAS Plus prior to acquisition on-board the instrument.)

For novel projects please contact us prior to preparing your samples